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Goat Anti-Chicken IgY Antibody (H&L) [HRP], pAb

Synonyms Goat Anti-Chicken IgY [HRP] pAb;
Description This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with chicken IgY heavy and light chains.
Antigen Species Chicken
Immunogen Full-length Chicken IgY (purified)
Host Species Goat
Purification Immunoaffinity chromatography
Conjugation Horseradish Peroxidase (HRP)

Subclass Goat IgG
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 1 mg/ml.
Species Reactivity Chicken
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

GenScript Goat Anti-Chicken IgY (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:30,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized
Technical Manual ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)


Deyuan Li.,et al. Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens.Clin Vaccine Immunol.2011Sep;18(9):1497 - 1502

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产品编号: A00165
规格
价格
数量
1 mg ¥790