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Goat Anti-Human IgG Antibody (H&L) [HRP], pAb

Synonyms Goat Anti Human IgG (H&L) [HRP]; Anti Human IgG (H&L) [HRP]
Description This polyclonal goat antibody is purified by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It binds to both the heavy and light chains of human IgG.
Antigen Species Human
Immunogen Full length human IgG (purified)
Host Species Goat
Purification Immunoaffinity chromatography
Conjugation Horseradish Peroxidase (HRP)

Subclass Goat IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal
Species Reactivity Human
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

GenScript Goat Anti-Human IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000.
Western blot: 1:5,000-1:50,000
Other applications: user-optimized
Technical Manual ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)




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产品编号: A00166
规格
价格
1 mg ¥790