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HA-tag Antibody, pAb, Goat

Synonyms Goat Anti HA-tag; Anti HA-tag
Description Goat Anti-HA-tag Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography.
Immunogen YPYDVPDYA (influenza hemagglutinin-HA (epitope)) conjugated to KLH
Host Species Goat
Purification Immunoaffinity chromatography
Conjugation Unconjugated

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity This antibody specifically reacts with HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescence (IF): 2-10 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other applications: user-optimized
Technical Manual ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)


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产品编号: A00168
规格
价格
40 ug ¥990