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HA-tag Antibody [HRP], pAb, Goat

Synonyms Anti HA-tag [HRP];
Description Goat Anti-HA-tag [HRP] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Goat Anti-HA-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain a HA-tag.
Antigen Species Influenza Virus
Immunogen YPYDVPDYA (influenza hemagglutinin-HA (epitope)) conjugated to KLH
Host Species Goat
Purification Immunoaffinity chromatography
Conjugation Horseradish Peroxidase (HRP)

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5mg/ml.
Specificity This antibody specifically reacts with HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)


Zhou X, et al. MKP-1 inhibits high NaCl-induced activation of p38 but does not inhibit the activation of TonEBP/OREBP: opposite roles of p38alpha and p38delta. Proc. Natl. Acad. Sci. U.S.A. Apr. 8 2008;105(14):5620-5.

Chen L.,et al. Sumoylation Regulates Nuclear Localization and Function of Zinc Finger Transcription Factor ZIC3.Biochim Biophys Acta.2013Jul;

I Beketaev, et al. Potentiation of Tbx5-mediated transactivation by SUMO conjugation and Protein Inhibitor of Activated STAT 1 (PIAS1). Int J Biochem Cell Biol. 2014Feb.;

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产品编号: A00169
规格
价格
数量
40 ug ¥990