您的位置 :首页 >> 产品 >> 抗体产品>>c-Myc-tag Antibody, pAb, Rabbit

c-Myc-tag Antibody, pAb, Rabbit

Synonyms Rabbit Anti-c-Myc-tag pAb;
Description The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Immunogen c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Host Species Rabbit
Purification Immunoaffinity chromatography
Conjugation Unconjugated

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
Specificity This Antibody recognizes c-Myc tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunofluorescence: 5-20 µg/ml
Immunoprecipitation (IP): 2-5 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
IF & ICC Protocol (pdf)


Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;

Shuai Chen.,et al. Enhancer of Zeste Homolog 2 Is a Negative Regulator of Mitochondria-Mediated Innate Immune ResponsesJ Immunol.2013Aug;

Yu-Rong Li.,et al. Hpa2 Is Required by HrpF to Translocate Xanthomonas oryzae TAL Effectors into Rice for Pathogenicity.Appl Environ Microbiol.2011Jun;

Zhang B.,et al. The p16-Specific Reactivation and Inhibition of Cell Migration Through Demethylation of CpG Islands by Engineered Transcription Factors.Hum Gene Ther.2012Otc;23(10):1071-1081

Anastasios Georgakopoulos.,et al. Presenilin1/γ-secretase promotes the EphB2-induced phosphorylation of ephrinB2 by regulating phosphoprotein associated with glycosphingolipid-enriched microdomains/Csk binding protein.FASEB J.2011Otc;25(10):3594 - 3604

Zhenhua Zheng.,et al. Enterovirus 71 2C Protein Inhibits TNF-αmediated Activation of NF-κB by Suppressing IκB Kinase β Phosphorylation.J Immunol.2011Sep;187(5):2202 - 2212

Xu ZY.,et al. Cold-inducible expression of AZI1 and its function in improvement of freezing tolerance of Arabidopsis thaliana and Saccharomyces cerevisiae.J Plant Physiol.2011Sep;168(13):1576-87

Ikuo Masuho.,et al. Type 5 G Protein β Subunit (Gβ5) Controls the Interaction of Regulator of G Protein Signaling 9 (RGS9) with Membrane Anchors.J Biol Chem.2011Jun;286(24):21806 - 21813

请点击此处查找更多相关文献
产品编号: A00172
规格
价格
数量
40 ug ¥790