Specificity |
GenScript TSH Antibody (3B12D11), mAb, Mouse detects endogenous levels of human TSH and does not cross-react with human HCG. |
Host Species |
Mouse |
Immunogen |
purified TSH from human pituitary |
Species Reactivity |
Human. Reactivity to other species is not tested yet. |
Conjugate |
Unconjugated |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
Application |
Recommended Usage |
Sandwich ELISA |
Capture: 0.5-10 µg/ml
Detection: 0.05-0.2 µg/ml |
|
Recommended antibody pairing for sandwich immunoassay: These antibodies are perfect choice for in vitro diagnostic assay development. And they are prepared for non-clinical research use only. The recommended pairs are based on our laboratory results.
|
Capture |
Detection |
A01709 (1H4G9) |
A01710 (2D10F6) |
A01711 (2B9H4) |
A01712 (26A7D2) |
A01713 (28F7B8) |
A01714 (3B12D11) |
A01709 (1H4G9) |
|
- |
- |
+++ |
- |
- |
A01710 (2D10F6) |
- |
|
- |
++ |
- |
- |
A01711 (2B9H4) |
- |
- |
|
- |
+ |
- |
A01712 (26A7D2) |
+ |
+ |
+ |
|
- |
++ |
A01713 (28F7B8) |
- |
- |
+++ |
- |
|
- |
A01714 (3B12D11) |
- |
- |
- |
- |
- |
|
The above data was achieved by Sandwich ELISA. ‘+’ means reaction and ‘-‘means no reaction.
The number of ‘+’ represents reaction intensity. |
Form |
Lyophilized |
Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Products of 1mg and 5mg size are provided in liquid form. |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
Storage Instructions |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Purification |
Protein A affinity column |
Isotype |
Mouse IgG1,κ |
Clonality |
Monoclonal |
Clone ID |
3B12D11 |
Target Background |
Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit and a unique beta subunit that confers specificity. Free alpha and beta subunits have essentially no biological activity. TSH (Thyroid stimulating hormone) is secreted from cells in the anterior pituitary and it is indispensable for the control of thyroid structure and metabolism. Free alpha and beta subunits have essentially no biological activity.GenScript TSH Antibody (3B12D11), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified TSH from human pituitary. |
Synonyms |
FSHA antibody; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.