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Human VISTA Antibody (VA.F6), mAb, Mouse

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The product is specific for human VISTA (UniProt Accession: Q9H7M9; Gene ID: 64115). This antibody binds with recombinant VISTA protein in ELISA and human VISTA expressing cell line in flow cytometry assay.
A01864
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Specificity The product is specific for human VISTA (UniProt Accession: Q9H7M9; Gene ID: 64115). This antibody binds with recombinant VISTA protein in ELISA and human VISTA expressing cell line in flow cytometry assay.
Host Species Mouse
Immunogen Recombinant human VISTA-Fc
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 µg/ml
Flow Cytometry 2-10 µg/ml

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. GenScript can customize this product per customer's request including product size, buffer components, etc.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID VA.F6

Target Background VISTA is a negative immune checkpoint regulator that suppresses T-cell activation, and highly expressed on hematopoietic cells and within the tumor microenvironment. By analogy to VISTA and PD-L1 blockade, VISTA blockade may offer an immunotherapeutic strategy for human cancer. Therapeutic antibody against VISTA activates T cell signal and offers a way to promote this blockade.GenScript Human VISTA Antibody (VA.F6), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with recombinant human VISTA.
Synonyms Mouse monoclonal to VSIR/V-set immunoregulatory receptor/B7H5/ GI24/ B7-H5/ PD-1H/ SISP1/ PP2135/ C10orf54/ DD1alphaPDCD1 Homolog/V-Set Domain-Containing Immunoregulatory Receptor/V-Domain Ig Suppressor Of T Cell Activation / Chromosome 10 Open Reading Fr

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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