| Specificity |
The product is specific for Francisella tularensis subsp. novicida U112 Cpf1. This antibody binds with recombinant Francisella tularensis subsp. novicida U112 Cpf1 protein in ELISA and Endogenous overexpressed Francisella tularensis subsp. novicida U112 Cpf1 in immunofluorescence, western blot. |
| Host Species |
Mouse |
| Immunogen |
Recombinant Francisella tularensis subsp. novicida U112 Cpf1 (FnCpf1) |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.005-1 µg/ml |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
0.2-1 µg/ml |
| Western Blot |
1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, contains 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b,κ |
| Clonality |
Monoclonal |
| Clone ID |
9H6 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
| Target Background |
Cpf1-containing CRISPR-Cas loci of Francisella tularensis subsp. novicida U112 encode functional defense systems capable of mediating plasmid interference in bacterial cells guided by the CRISPR spacers. Unlike Cas9 systems, Cpf1-containing CRISPR systems have three features: First, Cpf1-associated CRISPR arrays are processed into mature crRNAs without the requirement of an additional trans-activating crRNA (tracrRNA). Second, Cpf1-crRNA complexes efficiently cleave target DNA proceeded by a short T-rich protospacer adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9 systems. Third, Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. |
| Synonyms |
Mouse monoclonal to FnCpf1/Strain U112 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
