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Interleukin-8 (IL-8, 77aa)/CXCL8, Human



Synonyms CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), NAP-1
Description Interleukin-8 is one of the first discovered chemokines and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: a 77 a.a. protein produced by endothelial cells, and the more active 72 a.a. protein produced by monocytes. The receptors for IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cell morphology, activate integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells.
Recombinant human Interleukin-8 (IL-8, 77aa)/CXCL8 produced in E. coli is a single non-glycosylated polypeptide chain containing 77 amino acids. A fully biologically active molecule, rhIL-8(77aa)/CXCL8 has a molecular mass of 8.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P14210
Species Human
Source E. coli
Specific Activity The EC50 value of human IL-8(77aa) on Ca2+ mobilization assay in CHO-K1/G15/hCXCR1 cells (human Ga15 and human CXCR1 stably expressed in CHO-K1 cells) is less than 150 ng/ml.
Sequence
AVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKE
NWVQRVVEKFLKRAENS

Measured Molecular Weight 8.9 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Interleukin-8 (IL-8)(77aa)/CXCL8 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, recombinant human Interleukin-8 (IL-8)(77aa)/CXCL8 should be stable up to 1 week at 4°C or up to 2 months at -20°C.





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产品编号: Z03262
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1 mg ¥10800
25 ug ¥700
5 ug ¥300