快速单克隆抗体制备服务

为满足客户有时对单克隆抗体的迫切需求,金斯瑞快速单克隆抗体制备服务可在60天内完成单抗制备过程。我们通过专利的OptimunAntigenTM 抗原设计软件、自主研发的专利佐剂以及纳米抗原制备技术等,保证为客户提供2个阳性克隆以及高亲和力的抗体产品。

金斯瑞专利的OptimumAntigenTM 抗原设计软件、自主研发的专利佐剂以及纳米抗原制备技术,保证了快速单克隆抗体制备服务能在60天以内为客户提供高质量的杂交瘤细胞,针对蛋白抗原单抗服务,我们还承诺,免疫原WB检测阳性。

快速单克隆抗体制备服务


周期快:完成杂交瘤制备只需45天,节省时间
服务全面:客户可依据个人情况,自由选择蛋白抗原或使多肽抗原制备单抗
品质高:抗体亲和力高,特异性好
宿主选择:小鼠或大鼠可供选择,可避免因抗原同源性而造成的免疫耐受
生产规范: 拥有AAALAC和OLAW认证,能够从事大规模快速单抗制备

  • MonoExpress™单抗服务
  • 服务选择指南
  • 客户科研成果
  • 实验数据

蛋白抗原单抗服务套餐

蛋白抗原服务套餐
承诺
原始材料
免疫动物
客户筛选
交付内容
首次交付时间
MonoExpress™ Premium
SC1572P
MonoExpress™ Premium SC117
  • 至少1个克隆WB检测阳性
  • ELISA ≥ 1:64,000
  • 金斯瑞制备的抗原蛋白
  • 客户提供:靶蛋白名称,序列(如有NCBI 序列号,请一并提供)
5只Balb/c小鼠或3只大鼠
10个上清液用于评估
  • 最多5个杂交瘤
  • 2 mg纯化抗体(选择一株杂交瘤细胞)
  • 200 μg免疫原蛋白
11 周
MonoExpress™ Basic
SC1572
MonoExpress™ Basic SC1717
  • 免疫原WB检测阳性
  • ELISA ≥ 1:64,000
使用客户提供的蛋白作为免疫原
5只Balb/c小鼠或3只大鼠
5个上清液用于评估
  • 最多5个杂交瘤
  • 2 mg纯化抗体(选择一株杂交瘤细胞)
6-7 周


多肽抗原单抗服务套餐

多肽抗原服务套餐
服务优势及承诺
原始材料
免疫动物
交付内容
交付时间
MonoExpress™ Gold
SC1710
3 Peptide Antigens
  • 承诺免疫原ELISA检测阳性
  • 使用三条多肽制备杂交瘤,制备至少一个有效抗体的成功率高达95%以上
 
  • 金斯瑞合成多肽作为免疫原
  • 客户提供:靶蛋白名称,序列(如有NCBI 序列号,请一并提供)
15只Balb/c 小鼠 或 9只大鼠 (每条多肽免疫5只Balb/c 小鼠 或3只大鼠)
  • 6株杂交瘤细胞(2个克隆/ 每条多肽)
  • 6 个上清 (5 ml/ 每个克隆)
  • 2 mg未偶联多肽
13 周
MonoExpress™ Silver
SC1661
2 Peptide Antigens
  • 承诺免疫原ELISA检测阳性
  • 使用两条多肽制备杂交瘤,制备至少一个有效抗体的成功率高达85%以上
10只Balb/c 小鼠 或6只大鼠 (每条多肽免疫5 只Balb/c 小鼠 或3只大鼠)
  • 4株杂交瘤细胞(2个克隆/ 每条多肽)
  • 4个上清 (5 ml/ 每个克隆)
  • 2 mg未偶联多肽
MonoExpress™ Bronze
SC1660
1 Peptide Antigen
  • 承诺免疫原ELISA检测阳性
  • 使用1条多肽作为免疫原,已成功制备数百个抗体
5只Balb/c 小鼠 或3只大鼠
  • 2株杂交瘤细胞(2个克隆/ 每条多肽)
  • 2个上清 (5 ml/ 每个克隆)
  • 2 mg未偶联多肽

如果这些标准化套餐无法满足您的需求,我们还为您准备了定制化单抗服务套餐,满足您的个性化需求。

抗原种类

What kind of antigen

使用快速单抗服务客户发表的文章

Full List

  • Binici J, Hartmann J, Herrmann J, Schreiber C, Beyer S, GÜler G, Vogel V, Tumulka F, Abele R, Mäntele W, Koch JA soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is essential and sufficient for inhibition of NKp30-dependent cytotoxicity of natural killer cells. J Biol Chem. Oct. 2013; jbc.M113.483602
  • Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W, Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen G, Arrington CB, Annaert W. Rer1p maintains ciliary length and signaling by regulating-secretase activity and Foxj1a level. J Cell Biol. Mar. 2013; 6: 709-720
  • Zhu L, Liu D, Li Y, Li N. Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiol. Feb. 2013; 2: 904-917 Moh MC, Lorenzini PA, Gullo C, Schwarz H. Tumor
  • Moh MC, Lorenzini PA, Gullo C, Schwarz H.Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.FASEB J. Apr. 2013; 27, 2957-2966
  • Azevedo MF, Sanders PR, Krejany E, Nie CQ, Fu P, Bach LA, Wunderlich G, Crabb BS, Gilson PR. Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development.Biochem J. Jun 2013; 452, 433-441
  • Nutabi Camargo, Jos F. Brouwers, Maarten Loos, David H. Gutmann, August B. Smit, and Mark H. G. Verheijen.High-Fat Diet Ameliorates Neurological Deficits Caused By Defective Astrocyte Lipid Metabolism. FASEB J. Oct. 2012; 10, 4302-4315
  • Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin Hao. Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced Anthocyanin Biosynthesis And Red Fruit Coloration In Apple. Plant Physiol. Oct. 2012; 160, 1011-1022
  • Lacroix-Pépin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA. The Multidrug Resistance-Associated Protein 4 (Mrp4) Appears As A Functional Carrier Of Prostaglandins Regulated By Oxytocin In The Bovine Endometrium. Endocrinology. Dec. 2011; 152, 4993-5004

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibody generated by conventional immunization protocol had weak specificity to acetylated peptide antigens and cross-reactivity with corresponding non-acetylated peptide antigens.

MonoExpress™ Protocol

MonoExpress™ antibodies had high specificity to acetylated peptide antigens and no cross-reactivity with corresponding non-acetylated peptide antigens.

Indirect ELISA screen of hybridoma supernatants generated by MonoExpress™ vs. conventional immunization protocols

Conventional Protocol

The antibodies generated by conventional immunization protocol each having a weak binding specificity is shown:

  • Clone 1: Recognized dual phosphorylated peptide, single phosphorylated peptide 1, single phosphorylated peptide 2 and non-phosphorylated peptide.
  • Clone 2: Recognized dual phosphorylated peptide, single phosphorylated peptide 1 and single phosphorylated peptide 2.

MonoExpress™ Protocol

A panel of monoclonal antibodies each having a unique binding specificity is shown:

  • Clone 1: Only recognized dual phosphorylated peptide.
  • Clone 2: Only recognized dual phosphorylated peptide.
  • Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1.
  • Clone 4: and Clone 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2.

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services

monoexpress antibody

Affinity analysis of a monoclonal antibody generated by MonoExpress™ Antibody Services.
Biacore antibody/antigen affinity analysis showed that the MonoExpress™ generated antibody exhibited an equal or greater affinity to its complementary antigen, compared to an antibody generated by conventional immunization. Typically monoclonal antibodies generated by MonoExpress™ services display reasonably high affinities to their target antigens.

 

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