快速多抗服务

PolyExpressTM快速兔多克隆抗体服务

PolyExpressTM快速多克隆抗体服务是金斯瑞公司综合抗原设计软件、佐剂和独家免疫方案后全新推出的快速抗体制备服务。我们的服务可接受客户提供的抗原,也可以帮助客户准备抗原, 45个自然日完成多克隆抗体生产,承诺最终ELISA效价大于1:64,000,蛋白抗原更承诺Western blot检测结果阳性。

服务特点

  • 速度快:45个自然日即可交付定制的抗体
  • 客户可提供抗原,也可以请金斯瑞帮忙合成多肽抗原,或表达蛋白抗原
  • 效率高:我们自主研发的佐剂实验效果是弗氏佐剂的两倍
  • 质量保证:每个项目均严格参照SOP规定流程执行,确保实验质量
  • 附加服务免费:可帮助客户免费设计多肽抗原,免费偶联
  • 确保免疫应答:金斯瑞承诺最终ELISA效价大于1:64,000
  • 蛋白抗原承诺Western blot检测结果阳性

全新的抗体技术服务

金斯瑞搬迁至江宁新址后,配备了设施先进且完善的动物房,再加上不断提高的技术人员的服务水平,抗体服务生产能力显著上升。

  • 占地4,500,000 ft2恒温恒湿动物房
  • AAALAC 及OLAW国际认证
  • 动物房管理人员均受过很好的培训,经验丰富
  • 所有流程均严格按照SOP执行
  • 饲养动物种类齐全,包括兔、小鼠、大鼠
  • 金斯瑞合成/表达抗原
  • 客户提供抗原
  • 服务选择指南
  • 客户科研成果
  • 案例
蛋白抗原
PolyExpress™
Gold Plus
PolyExpress™ Premium
PolyExpress™ Premium Plus
使用重组蛋白定制抗体**
protein antigen polyclonal antibodies
40 ml 抗血清
40 ml antiserum
2个亲和纯化抗体(0.5-3 mg/个)及200 ug蛋白抗原
affinity-purified pAb
1个亲和纯化抗体(1-5 mg)及1 mg蛋白抗原
affinity-purified pAb
Guaranteed to Perform in Antigen Western Blot and ELISA applications
多肽抗原
PolyExpress™
Basic
PolyExpress™
Silver
PolyExpress™
Gold
针对内源性蛋白设计1条抗原

1 Peptide Antigen
针对内源性蛋白设计2条抗原

2 Peptide Antigens
针对内源性蛋白设计3条抗原

3 Peptide Antigens
针对内源性蛋白产生1个特异性抗体
1 Polyclonal Antibody Against 1 Epitope
针对内源性蛋白产生2个特异性抗体
2 Polyclonal Antibodies Against 2 Individual Epitopes2 Polyclonal Antibodies Against 2 Individual Epitopes

针对内源性蛋白产生3个特异性抗体
3 Polyclonal Antibodies Against 3 Individual Epitopes3 Polyclonal Antibodies Against 3 Individual Epitopes3 Polyclonal Antibodies Against 3 Individual Epitopes
数以千计的成功案例,质量保证
85%以上抗体WB检测结果阳性
95%以上抗体WB检测结果阳性
承诺1-5 mg 纯化抗体
2 个亲和纯化抗体 (1-5 mg/个)
3 个亲和纯化抗体 (1-5 mg/个)

*Silver及Gold服务套餐仅针对单个的靶蛋白序列,不适用于不同蛋白序列合并的序列

** 抗原蛋白依据方案选择,可能是蛋白全序列或是≥ 10 KD的蛋白片段

应用选择指南

Assays Western Blot Sandwich ELISA IF, IHC,
Flow Cytometry
IP ChIP
需求&级别
  • 一般亲和力及特定识别
  • 高特异性及灵敏度
  • 可用相同的多抗做捕获及检测抗体
  • 高特异性及灵敏度
  • 构象表位识别
  • 多个表位识别
  • 构象表位识别
Premium Plus
Premium
Gold Plus
Gold
Silver
Basic
PolyExpress Antigen-Specific Affinity Purified pAb(Protein,His-tag or Tag free)
PolyExpress Antigen-Specific Affinity Purified pAb(Protein,GST-Tag)
PolyExpress Protein A Purified pAb(Protein Antigen)
使用客户提供蛋白抗原(His-tag或tag free, 4-4.5 mg蛋白,浓度>0.4 mg/ml,纯度>85%)
定制抗体
Recombinant Protein as Antigen

使用客户提供蛋白抗原(GST-Tag,总分子量>50 kD, 4-4.5 mg蛋白,浓度>0.4 mg/ml,纯度>85%)定制抗体
Recombinant Protein as Antigen

使用客户提供蛋白抗原(2-2.5 mg蛋白,浓度>0.4 mg/ml,纯度>85%)定制抗体
Recombinant Protein as Antigen
免疫两只兔子
免疫两只兔子
免疫两只兔子
1-5 mg亲和纯化抗体
3 Polyclonal Antibodies Against 3 Individual Epitopes

0.2-2 mg亲和纯化抗体(去除Tag的交叉反应)
2 Polyclonal Antibodies Against 2 Individual Epitopes


50-100 mg Protein A纯化抗体
1 Polyclonal Antibody Against 1 Epitope
承诺最终ELISA效价大于1:64,000,免疫原WB阳性
Protein versus peptide antigen custom polyclonal antibody selection guide

使用金斯瑞快速多抗服务发表的部分客户文献

  • Jiang L, Mu J, Zhang Q, Ni T, Srinivasan P, Rayavara K, Yang W, Turner L, Lavstsen T, Theander TG, Peng W, Wei G, Jing Q, Wakabayashi Y, Bansal A, Luo Y, Ribeiro. PfSETvs methylation of histone H3K36 represses virulence genes in Plasmodium falciparum. Nature, Jul. 2013; 499, 223-227
  • Sebastian S, Faralli H, Yao Z, Rakopoulos P, Palii C, Cao Y, Singh K, Liu QC, Chu A, Aziz A, Brand M, Tapscott SJ, Dilworth FJ. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes Dev. Jun. 2013; 27, 1247-1259
  • Stuart Cane, Anna A. Ye, Sasha J. Luks-Morgan, and Thomas J. Maresca. Elevated polar ejection forces stabilize kinetochore–microtubule attachments. J Cell Biol. Jan. 2013; 200, 203-218
  • Wang P, Galan JA, Normandin K, Bonneil E, Hickson GR, Roux PP, Thibault P, Archambault V. Cell cycle regulation Greatwall kinase nuclear localization facilitates mitotic progression. J Cell Biol. Jul. 2013; 202, 277-293
  • Lechtreck KF, Brown JM, Sampaio JL, Craft JM, Shevchenko A, Evans JE, Witman GB. Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase. J Cell Biol. Apr. 2013; 201, 249-261
  • Kim DH, Sung S. Coordination of the Vernalization Response through a VIN3 and FLC Gene Family Regulatory Network in Arabidopsis. Plant Cell. Feb. 2013; 25, 454-469

客户成功案例

Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.

Cost-effective Custom Polyclonal Antibodies Services

The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)

J Lee, M Song, S Hong. Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration. PLoS Genet. June 2013, 9, e1003559

Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.

ELISA

ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.


ELISA titer guaranteed custom polyclonal antibody services

ELISA analysis of purified antibody generated by PolyExpress™ Antibody Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.

订购信息

Order online 订单下载
Order by mail 邮箱: antibody@genscript.com.cn
Order by phone 电话: 400-025-8686-5811

 

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