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Plant carbonic anhydrase-like enzymes in neuroactive alkaloid biosynthesis

Nature. 2023-11; 
Ryan S Nett, Yaereen Dho, Chun Tsai, Daria Passow, Jaime Martinez Grundman, Yun-Yee Low, Elizabeth S Sattely
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摘要

Plants synthesize numerous alkaloids that mimic animal neurotransmitters. The diversity of alkaloid structures is achieved through the generation and tailoring of unique carbon scaffolds, yet many neuroactive alkaloids belong to a scaffold class for which no biosynthetic route or enzyme catalyst is known. By studying highly coordinated, tissue-specific gene expression in plants that produce neuroactive Lycopodium alkaloids, we identified an unexpected enzyme class for alkaloid biosynthesis: neofunctionalized α-carbonic anhydrases (CAHs). We show that three CAH-like (CAL) proteins are required in the biosynthetic route to a key precursor of the Lycopodium alkaloids by catalysing a stereospecific Mannich-like c... More

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