4X LDS Sample Buffer is used to prepare protein samples for denaturing polyacrylamide gel electrophoresis (PAGE) with SurePAGE™, ExpressPlus™ and most other types of Bis-Tris gels. LDS sample buffer contains lithium dodecyl sulfate with pH at 8.4, which helps reducing the disulfide bonds and ensure optimal protein separation.
5X Sample Buffer is used as a tracking dye for SDS PAGE gel loading. This buffer contains SDS and is suitable for denaturing gel electrophoresis. Dilute 1:4 with sample before loading. Note: 5X Sample Buffer should be brought to room temperature prior to use. Mix well before loading gel.