Specificity |
Anti-c-Myc Tag Antibody (9E10), mAb, Mouse is specific for Myc tagged proteins. |
Host Species |
Mouse |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
Application |
Recommended Usage |
Western Blot |
1:500-1:2,000 |
Immunohistochemistry (IHC) |
1:50-1:1,000 |
Immunocytochemistry/Immunofluorescence (ICC/IF) |
1:100-1:500 |
Flow Cytometry |
1:50-1:200 |
Immunoprecipitation (IP) |
1:100-1:500 |
ELISA |
1:1000-1:10000 |
|
Form |
Liquid |
Storage Buffer |
Supplied in PBS (pH 7.2), containing 0.03% Proclin 300 and 50% glycerol |
Concentration |
1.0 mg/ml |
Storage Instructions |
The product remains stable up to 1 year at -20 °C from date of receipt. Avoid repeated freezing and thawing cycles. |
Purification |
Protein A affinity column |
Isotype |
Mouse IgG1 |
Clonality |
Monoclonal |
Clone ID |
9E10 |
Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Target Background |
The c-Myc tag is a small peptide tag with amino acid sequence of EQKLISEEDL. It is derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. c-Myc Tag Antibody (9E10) is a famous mouse monoclonal clone for the analysis of c-Myc tagged recombinant protein. The 9E10 clone has been cited in more than 9000 publications, widely used in various applications, such as Western Blot (WB), Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF) and Immunohistochemistry (IHC) and so on. GenScript Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (Cat.No.: A02300) is a recombinant antibody, which has several advantages including: good batch-to-batch consistency, high reproducibility, comparative sensitivity and specificity, and animal-free production |
Synonyms |
c-myc Antibody; c-myc tag antibody, Myc Epitope Tag antibody |
For research use only. Not intended for human and animal therapeutic or diagnostic use.