| Product Description |
Human Recombinant CHO-K1/MC4/Gα15 (cAMP) Stable
Cell Line stably overexpress the melanocortin 4 receptor gene (MC4). This stable cell line product is designed for
measuring the In Vitro function of MC4. |
| Applications |
Functional assay for MC4 receptor (cAMP accumulation assay)
|
| Expressed Gene |
Codon optimized from NM_005912;
no expressed tags
|
| Target Protein |
NP_005903
|
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen cells (1×106 per
vial in 1 mL)
|
| Storage |
Store cells in liquid nitrogen
immediately upon receipt. Thaw and recover cells within one year from the date
received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat.
No. LT07-318,
Lonza). |
| Stability |
Stable through more than 16 passages
without significant changes in assay performance or expression profile. |
| Culture Properties |
Adherent
|
| Freeze Medium |
45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco),
45% FBS (Cat. No. A5669701,
Gibco), 10%
DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml puromycin (Cat. No. A11138-03, Life
Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Gene Synonyms |
BMIQ20, MC4R
|
| Background |
The melanocortin family consists of five melanocortin
G-Protein-coupled receptors (designated MC1-5R). The melanocortins, α-, β- and
γ-melanocyte-stimulating hormones (MSHs), are peptides derived from a precursor
protein POMC, and play important roles in energy balance, reproductive
function, pigmentation and inflammation. MC4 receptors with high affinities for
α-MSH and ACTH are expressed almost exclusively in the CNS and appear to play a
prominent role in energy homeostasis. |
Figure 1: NDP-α-MSH-induced concentration-dependent stimulation of cAMP in CHO-K1/MC4/Gα15 (cAMP) cells. d2 acceptor fluorophore (Cat. No. 62AM4PEC, Cisbio)-labeled cAMP and intracellular cAMP in CHO-K1/MC4/Gα15 (cAMP) cells competitively bind with Europium Cryptate -labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by Varioskan LUX (3020, Thermo). The EC50 of NDP-α-MSH in CHO-K1/MC4/Gα15 (cAMP) cells was 9.99 nM.
For research use only. Not intended for human or animal
clinical trials, therapeutic or diagnostic use.
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