| Species |
Rat |
| Protein Construction |
IL-3β (Met26-Cys169)
Accession # P97688 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
The ED50 as determined by a cell proliferation assay using murine MC-9 cells is less than 10.0 ng/ml, corresponding to a specific activity of 1.0 × 105IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
16.3 kDa |
| Formulation |
Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. It exerts its biological activities through binding to Interleukin-3 receptors included α and β subunits, but sequence analysis revealed a number of features indicative of the presence of only one β-subunit in the rat. Interleukin-3 acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Furthermore, rat and human IL-3 share low homology and have not cross species activity. |
| Synonyms |
Hematopoietic Growth Factor; MCGF; Multipotential Colony-stimulating Factor; P-cell-stimulating Factor |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
