| Species |
Salmon |
| Protein Construction |
Expressed with an N-terminal Met.
IGF-1 (Gly45-Ala114)
Accession # Q02815 |
|
| Purity |
≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 15.0 ng/ml as determined by a dose-response proliferation assay using FDC-P1 cells. Based on the ED50, the calculated specific activity is approximately >6.7 × 104 units/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
7.7 kDa |
| Apparent Molecular Weight |
~8.0 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in H2O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C. |
The ED50 as determined by a cell proliferation assay using FDC-P1 cells is 7.2 ng/ml.
Lane 1: 2 µg of IGF-I, Salmon, reducing (R)
The purity is greater than 95% as analyzed by SDS-PAGE
| Target Background |
Insulin-like growth factor 1 (IGF-1), also called Somatomedin, is a hormone similar in molecular structure to insulin but has a much higher growth-promoting activity. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. It is able to stimulate glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. It may also play a role in synapse maturation. |
| Synonyms |
Somatomedin; IGF1; Insulin-like growth factor I; IGF-1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
