Species |
SARS-CoV-2 |
Protein Construction |
Expressed with the mutations of D63G, R203M, D377Y.
N protein (Met 1 - Ala 419) Accession # P0DTC9 |
Poly-His |
N-term |
C-term |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Biological Activity |
SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag can bind with SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (Cat. No. A02135) in functional ELISA assay. |
Expression System |
E.coli |
Theoretical Molecular Weight |
46.4 kDa |
Apparent Molecular Weight |
~47 kDa, on SDS-PAGE under reducing conditions. |
Formulation |
Supplied as a solution of 50 mM Tris-HCl, 150 mM NaCl, pH 8.0, containing 300 mM Imidazole |
Concentration |
Please refer to the COA for the specific lot |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Please avoid repeated freeze-thaw cycles |
![SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag](/product/documents/down?doc_name=Z03732_SDS PAGE.JPG&file=scm_files/productFile_notes/2021/12/14/b7036c0a-a3e4-4d09-b394-735a1bbec658.JPG)
Lane R: 2 μg of SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag, reducing(R)
> 90% as analyzed by SDS-PAGE
![SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag](/product/documents/down?doc_name=Z03732_ELISA.jpg&file=scm_files/productFile_notes/2021/12/14/b9593621-461c-439f-a1a7-f950d504735c.jpg)
ELISA binding of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) with SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag (GenScript, Z03732)
Coating antigen:
SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag (GenScript, Z03732), 1 μg/mL
SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) dilution start from 2 μg/ml
Target Background |
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is the most abundant structural protein of the coronavirus which is associated with the nucleic acid. The sublineage B.1.617.2 has been redesignated as a "variant of concern" (VOC-21APR-02) in May 2021, which spreads more quickly than the original version of the virus. |
Synonyms |
Lineage B.1.617.2; VUI-21APR-02; variant Delta |
References |
1. Tracking SARS-CoV-2 variants.
2. What You Need to Know About the Delta Variant. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.