Products/Services Used | Details | Operation |
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Gene Synthesis> | ...With the advent of cheap and reliable gene synthesis technologies, we routinely use commercial providers, such as GenScript, to synthesize the gene targeting vectors which are subcloned into the AAV plasmid backbone containing the inverted terminal repeats... | Get A Quote |
The ability to edit the genome of cell lines has provided valuable insights into biological processes and the contribution of specific mutations to disease biology. These techniques fall into two categories based on the DNA repair mechanism that is used to incorporate the genetic change. Nuclease-based technologies, such as Zinc-Finger Nucleases, TALENS, and Crispr/Cas9, rely on non-homologous end-joining (NHEJ) and homology directed repair (HDR) to generate a range of genetic modifications. Adeno-Associated Virus (AAV) utilizes homologous recombination to generate precise and predictable genetic modifications directly at the target locus. AAV has been used to create over 500 human isogenic cell lines comprisin... More