Monoclonal antibodies (MAbs) are important tools for the study of proteins′ function and structure. But there has been no report as to the preparation of MAbs against human KIAA0100 protein up to date. Here, firstly, we generated the mouse MAb against human KIAA0100 protein using purified recombinant 6xHistidinc (6xHis)-tagged human KIAA0100 protein segment (1557-2234) as an antigen; secondly, the mRNA expression of human KIAA0100 gene was detected in U937 cells using Northern blot analysis. Our results showed that the mouse MAb against human KIAA0100 protein could sensitively recognize the human KIAA0100 protein using western blot analysis and immunocytochemistry analysis. Besides, western blot analysis ... More
Monoclonal antibodies (MAbs) are important tools for the study of proteins′ function and structure. But there has been no report as to the preparation of MAbs against human KIAA0100 protein up to date. Here, firstly, we generated the mouse MAb against human KIAA0100 protein using purified recombinant 6xHistidinc (6xHis)-tagged human KIAA0100 protein segment (1557-2234) as an antigen; secondly, the mRNA expression of human KIAA0100 gene was detected in U937 cells using Northern blot analysis. Our results showed that the mouse MAb against human KIAA0100 protein could sensitively recognize the human KIAA0100 protein using western blot analysis and immunocytochemistry analysis. Besides, western blot analysis revealed that human KIAA0100 gene possibly encoded two different protein products (254 kDa and <250 kDa) in U937 cells. Moreover, Northern blot analysis confirmed human KIAA0100 gene possibly produced two different mRNA products (6000bp-10000bp and 5000bp-6000bp) in U937 cells. Collectively, these results provide a basis for large-scale production of the MAb against human KIAA0100 protein, which will be useful for the study of human KIAA0100 protein′s function/structure and MAb-targeted drugs in future.
