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PCR Cloning and Subcloning> | ... Materials and Methods. Subcloning, expression and purification of recombinant proteins: The catalytic domain of hPDE4D (380-756, codon optimized) was synthesized and subcloned into expression vector pET15b (Genscript). ... | Get A Quote |
In the past decade, surface plasmon resonance (SPR) biosensor-based technology has been exploited more and more to characterize the interaction between drug targets and small-molecule modulators. Here, we report the successful application of SPR methodology for the analysis of small-molecule binding to two therapeutically relevant cAMP phosphodiesterases (PDEs), Trypanosoma brucei PDEB1 which is implicated in African sleeping sickness and human PDE4D which is implicated in a plethora of disease conditions including inflammatory pulmonary disorders such as asthma, chronic obstructive pulmonary disease and central nervous system (CNS) disorders. A protocol combining the use of directed capture using His-tagged PD... More