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Gene Synthesis> | ... FLAG-tagged KLF1 was synthesized (GenScript) and subcloned into pCL20c/Mp-ires-GFP to create a ... amplicon was gel extracted (QIAquick, Qiagen), A-tailed and inserted into pCR2.1 TOP/TA (Invitrogen) to create pCR2.1/pSaf and sequence verified (GenScript). A KpnI- ... | Get A Quote |
Long noncoding RNAs (lncRNAs) interact with other RNAs, DNA and/or proteins to regulate gene expression during development. Erythropoiesis is one developmental process that is tightly controlled throughout life to ensure accurate red blood cell production and oxygen transport to tissues. Thus, homeostasis is critical and maintained by competitive outcomes of pro- and anti-apoptotic pathways. LncRNAs are expressed during blood development; however, specific functions are largely undefined. Here, a culture model of human erythropoiesis revealed that lncRNA Fas-antisense 1 (Fas-AS1 or Saf) was induced during differentiation through the activity of essential erythroid transcription factors GATA-1 and KLF1. Saf was ... More