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PCR Cloning and Subcloning> | ... their interaction with the hSR. Experimental Methods. Materials. Human secretin, human glucagon, and secretin analogs 1–5 and 15–20 of greater than 95% purity were purchased from GenScript, USA. The SNAP-tag vector ... | Get A Quote |
The pleiotropic role of human secretin (hSCT) validates its potential use as a therapeutic agent. Nevertheless, the structure of secretin in complex with its receptor is necessary to develop a suitable therapeutic agent. Therefore, in an effort to design a three-dimensional virtual homology model and identify a peptide agonist and/or antagonist for the human secretin receptor (hSR), the significance of the primary sequence of secretin peptides in allosteric binding and activation was elucidated using virtual docking, FRET competitive binding and assessment of the cAMP response. Secretin analogs containing various N- or C-terminal modifications were prepared based on previous findings of the role of these domain... More