Products/Services Used | Details | Operation |
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Peptide Synthesis> | ... Peptides were derived from Genscript (USA) and applied either i) 30 minutes before and during corticosterone treatment (50 µM); or ii) only during the last 30 min of corticosterone ... peptide as a control. Peptides were derived from Genscript (USA) and applied during the last ... | Get A Quote |
Glucocorticoid hormones, via activation of their receptors, promote memory consolidation, but the exact underlying mechanisms remain elusive. We examined how corticosterone regulates AMPA receptor (AMPAR) availability in the synapse, which is important for synaptic plasticity and memory formation. Peptides which specifically block the interaction between N-Ethylmaleimide-Sensitive Factor (NSF) and the AMPAR-subunit GluA2 prevented the increase in synaptic transmission and surface expression of AMPARs known to occur after corticosterone application to hippocampal neurons. Combining a live imaging Fluorescence Recovery After Photobleaching (FRAP) approach with the use of the pH-sensitive GFP-AMPAR tagging reveale... More