Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | ... The corresponding amplified product was directly ligated using the ClonEZ PCR cloning kit (Genscript) into the pETPhos plasmid [11], a variant of pET15b (Novagen) that includes a tobacco etch virus (TEV) protease site instead of the thrombin site, and a N-terminal His-tag free ... | Get A Quote |
Due to the emergence of methicillin-resistant strains, Staphylococcus aureus has become as major public-health threat. Studies aimed at deciphering the molecular mechanism of virulence are thus required to identify new targets and develop efficient therapeutic agents. Protein phosphorylations are known to play key regulatory functions and their roles in pathogenesis are under intense scrutiny. Here we analyzed the protein tyrosine phosphatase PtpA of S. aureus, a member of the family of low molecular weight protein tyrosine phosphatases that are often secreted by pathogenic bacteria. We report for the first time that PtpA is phosphorylated in vitro by the S. aureus tyrosine kinase CapA1B2. A mass spectrometr... More