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Peptide Synthesis> | ... Initially, the signal peptide sequences were removed from transcripts cn56 and cn57. The transcripts were further optimized by GenScript USA Inc. based on the preferred E. coli codon usage and the optimized transcripts were synthesized as pUC57 plasmid inserts. ... | Get A Quote |
Cryptocaryonosis is a major problem for mariculture, and the absence of suitable sero-surveillance tools for the detection of cryptocaryonosis makes it difficult to screen Cryptocaryon irritans-infected fish, particularly asymptomatic fish. In this study, we proposed a serum-based assay using selected C. irritans proteins to screen infected and asymptomatic fish. Eight highly expressed genes were chosen from an earlier study on C. irritans expressed sequence tags and ciliate glutamine codons were converted to universal glutamine codons. The chemically synthesized C. irritans genes were then expressed in an Escherichia coli expression host under optimized conditions. Five C. irritans proteins were successful... More