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DNA Sequencing> | ... of EGFP insertion in vKS15-01-EGFP virus, the corresponding viral genomic region (nt 3242–3700) was amplified by RT-PCR using the primer pair, SVA-6757F and SVA-7191R (Table S1), and PCR product was submitted for DNA sequencing (GenScript sequencing facility ... | Get A Quote |
A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary b... More