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Gene Synthesis> | ... The P. aeruginosa gene PA2138 encoding PaeLigD was synthesized by the GenScript Corporation and cloned between the NdeI and BamHI of bacterial expression vector pET-16b that allows expression of the recombinant protein fused to a N-terminal (His) 10 -tag followed ... | Get A Quote |
Bacillus subtilis is one of the bacterial members provided with a nonhomologous end joining (NHEJ) system constituted by the DNA-binding Ku homodimer that recruits the ATP-dependent DNA Ligase D (BsuLigD) to the double-stranded DNA breaks (DSBs) ends. BsuLigD has inherent polymerization and ligase activities that allow it to fill the short gaps that can arise after realignment of the broken ends and to seal the resulting nicks, contributing to genome stability during the stationary phase and germination of spores. Here we show that BsuLigD also has an intrinsic 5'-2-deoxyribose-5-phosphate (dRP) lyase activity located at the N-terminal ligase domain that in coordination with the polymerization and ligase activi... More