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PCR Cloning and Subcloning> | ... The STAT1 sequence was designed to incorporate a SUMO-tag with an Ulp1-cleavage site. Similar constructs were designed for STAT3 and STAT5B. A summary of the constructs prepared is shown in Table 1. The molecular cloning was performed by GenScript. Table 1. ... | Get A Quote |
The development of STAT protein-specific inhibitors has been the focus of a number of drug discovery programs. STAT activation occurs through phosphorylation at the STAT SH2 domain, resulting in dimerization, translocation to the nucleus, and transcription of proliferative genes. Due to the functional significance of the SH2 domain in mediating multiple components of the STAT signalling cascade, many libraries of inhibitors have been designed to target the SH2 domain. This has triggered the requirement for effective high-throughput screening platforms for analyzing binding by larger chemical libraries to STAT proteins. Herein, we present strategies for the development of a high-throughput thermal denaturation-b... More