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Plasmid DNA Preparation> | ...Synthetic genes encoding Ra, Rb, Rc, and Rd (S1 Fig) were produced by GenScript,For insertion of Ra-d into pT7-5 [36] under the T7 promoter, Ra and Rb were excised from the Genscript plasmids with BamHI and HindIII, Rc was digested with SacI and HindIII ... | Get A Quote |
Are integral membrane protein-encoding mRNAs (MPRs) different from other mRNAs such as those encoding cytosolic mRNAs (CPRs)? This is implied from the emerging concept that MPRs are specifically recognized and delivered to membrane-bound ribosomes in a translation-independent manner. MPRs might be recognized through uracil-rich segments that encode hydrophobic transmembrane helices. To investigate this hypothesis, we designed DNA sequences encoding model untranslatable transcripts that mimic MPRs or CPRs. By utilizing in vitro-synthesized biotinylated RNAs mixed with Escherichia coli extracts, we identified a highly specific interaction that takes place between transcripts that mimic MPRs and the cold shock pro... More