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Gene Synthesis> | ... EGCase I (R. equi), EGCase II (R. equi) or EGALC (R. equi) as queries. A codon optimized sequence encoding the R. triatomea EGCase I ORF with a C-terminal His-tag was synthesized by Genscript. A version of R. triatomea ... | Get A Quote |
The biological function of glycosphingolipids (GSLs) is largely determined by their glycan headgroup moiety. This has placed a renewed emphasis on detailed GSL headgroup structural analysis. Comprehensive profiling of GSL headgroups in biological samples requires the use of endoglycoceramidases with broad substrate specificity and a robust workflow that enables their high-throughput analysis. We present here the first high-throughput glyco-analytical platform for GSL headgroup profiling. The workflow features enzymatic release of GSL glycans with a novel broad-specificity endoglycoceramidase I (EGCase I) from Rhodococcus triatomea, selective glycan capture on hydrazide beads on a robotics platform, 2AB-fluoresc... More