Products/Services Used | Details | Operation |
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WoLF PSORT> | ... possibility. WoLF PSORT Prediction (http://www.genscript.com/psort/wolf_ psort.html) was used to further predict the subcellular localization, which suggested that the most likely location was the chloroplast. LocTree3 ... | Get A Quote |
BACKGROUND: The marine diatom, Phaeodactylum tricornutum, has become a model for studying lipid metabolism and its triacylglycerol (TAG) synthesis pathway makes it an ideal target for metabolic engineering to improve lipid productivity. However, the genetic background and metabolic networks of fatty acid biosynthesis in diatoms are not well understood. Glycerol-3-phosphate acyltransferase (GPAT) is the critical enzyme that catalyzes the first step of TAG formation. So far, characterization of GPAT in marine microalgae has not been reported, especially at the level of comprehensive sequence-structure and functional analysis. RESULTS: A GPAT was cloned from P. tricornutum and overexpressed in P. tricornutum. Volu... More