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Peptide Synthesis> | ... 1B). The signal peptide from mouse IgG kappa (METDTLLLWVLLLWVPGSTGD) was in- cluded to enable secretion. Genes were codon optimized, synthesized, and cloned into the pIRES2-AcGFP1 plasmid (Clontech, Mountain view, CA) by GenScript (Piscataway, NJ). ... | Get A Quote |
Our aim was to identify conformational epitopes, recognized by monoclonal antibodies (mAbs) made against human (h) interferon (IFN)-γ. Based on the mAbs' (n = 12) ability to simultaneously bind hIFN-γ in ELISA, 2 epitope clusters with 5 mAbs in each were defined; 2 mAbs recognized unique epitopes. Utilizing the mAbs' lack of reactivity with bovine (b) IFN-γ, epitopes were identified using 7 h/bIFN-γ chimeras where the helical regions (A-F) or the C terminus were substituted with bIFN-γ residues. Chimeras had a N-terminal peptide tag enabling the analysis of mAb recognition of chimeras in ELISA. The 2 mAb clusters mapped to region A and E, respectively; the epitopes of several mAbs also involved... More