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Bacterial Protein Expression System> | ... An N-terminal six histidine-tagged LeuRS from M. tuberculosis 103 H37Rv, which was codon-optimized for E. coli (GenScript, Piscataway NJ, USA), was over- 104 expressed and purified according to Novagen (Madison, WI, USA) using an E. coli 105 BL21(DE3) T7 RNA ... | Get A Quote |
The recent development and spread of extensively drug-resistant and totally drug-resistant resistant (TDR) strains of Mycobacterium tuberculosis highlight the need for new antitubercular drugs. Protein synthesis inhibitors have played an important role in the treatment of tuberculosis (TB) starting with the inclusion of streptomycin in the first combination therapies. Although parenteral aminoglycosides are a key component of therapy for multidrug-resistant TB, the oxazolidinone linezolid is the only orally available protein synthesis inhibitor that is effective against TB. Here, we show that small-molecule inhibitors of aminoacyl-tRNA synthetases (AARSs), which are known to be excellent antibacterial protein s... More