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Molecular Biology Reagents> | ... kDa. The expression cassette, named 60EGP (Supplementary Data 1), was synthetized and directly cloned into the plasmid pET-21 a(+) using the enzymes BamHI and AatII by the company Genscript (Piscataway, NJ, USA). ... | Get A Quote |
An important aspect related to infectious pathogens is their exceptional adaptability in developing resistance, which leads to a perpetual challenge in the discovery of antimicrobial drugs with novel mechanisms of action. Among them, antimicrobial peptides (AMPs) stand out as promising anti-infective molecules. In order to overcome the high costs associated with isolation from natural sources or chemical synthesis of AMPs we propose the expression of Pa-MAP 2, a polyalanine AMP. Pa-MAP 2 was fused to an ELP-intein tag where the ELP (Elastin-like polypeptide) was used to promote aggregation and fast and cost-effective isolation after expression, and the intein was used to stimulate a controlled AMP release. For ... More