Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | ... to visualize the protein bands, while the other was electro-transferred to an Amersham Protran Premium 0.45-μm PVDF membrane (GE Healthcare) for Western blot detection of recombinant proteins using the mouse anti-His tag monoclonal antibody (GenScript) as described ... | Get A Quote |
Clip domain serine proteinases (ClipSPs) play an important role in the prophenoloxidase-activating (proPO) system. In the shrimp Penaeus monodon, the ClipSP PmClipSP2 has been previously shown to bind to microbial polysaccharides (LPS and β-1,3-glucan) and likely activates the proPO system. To reveal the binding site of the PmClipSP2 protein, the N-terminal clip domain (Clip-PmClipSP) and C-terminal SP domain (SP-PmClipSP2) were separately cloned. The recombinant proteins were then assayed for their binding properties and involvement in proPO activation. According to the ELISA-based binding assay, rSP-PmClipSP2, but not rClip-PmClipSP, can bind immobilized LPS and β-1,3-glucan as well as significantly activat... More