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Gene Synthesis> | ... Plasmids encoding αcat-VH and αcat-VT were also gifts from Alpha Yap's lab ( 29) and subsequently cloned with GFP tag to get GFP-αcat-VH and GFP-αcat-VT. Plasmid encoding GFP-VT was synthesized by GenScript Biotech (Piscataway, NJ). ... | Get A Quote |
The mechanical properties of the microenvironment play a large role in influencing cellular behavior. In particular, the tradeoff between substrate viscosity and elasticity on collective cell migration by adherent cells is highly physiologically relevant, but remains poorly understood. To investigate the specific effects of viscous substrates, we plated epithelial monolayers onto polydimethylsiloxane substrata with a range of viscosities and elasticities. We found that on viscoelastic substrates the monolayers underwent rapid and coordinated movement to generate cell-free areas. To understand the molecular mechanism of this coordinated movement, we imaged various structural and signaling proteins at cell-cell a... More