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Proteins, Expression, Isolation and Analysis> | … The expression and purification of His-tagged protein were performed as previously described (Soong et al., 2013). Lipopolysaccharide (LPS) was removed by the Endotoxin Removal Kit (GenScript), which can effective reduce LPS levels to less 0.1 EU/ml. Vaccination of Mice … | Get A Quote |
(Mtb) remains a global health challenge due to the limited efficacy of the Mtb vaccine in current use, Bacillus Calmette-Guérin (BCG). To date, there is no available vaccine for immunocompromised individuals. Thus, there is an urgent need to develop a new vaccine candidate which can induce mucosal immunity in hosts with different immune statuses. DnaK (HSP70) has been shown to induce protective immunity against Mtb infection when administered by DNA vaccine; however, the protection is inferior to that induced by the BCG vaccine. In our study, we vaccinated C57BL/6J mice with DnaK protein alone. Subcutaneous or intranasal vaccination with DnaK generated IFNγ-secreting CD4 T cells in the spleen, but... More