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Gene Synthesis> | … Constructs to express N-terminal Flag and glutathione S-transferase dual tagged versions of human MID1-Bbox1, residues 110–165 and human MID1-Bbox1-Bbox2, residues 110–214 were synthesised at GenScript, China and subcloned into pET24b (Merck) … | Get A Quote |
Expression of mutant Huntingtin (HTT) protein is central to the pathophysiology of Huntington's Disease (HD). The E3 ubiquitin ligase MID1 appears to have a key role in facilitating translation of the mutant HTT mRNA suggesting that interference with the function of this complex could be an attractive therapeutic approach. Here we describe a peptide that is able to disrupt the interaction between MID1 and the α4 protein, a regulatory subunit of protein phosphatase 2A (PP2A). By fusing this peptide to a sequence from the HIV-TAT protein we demonstrate that the peptide can disrupt the interaction within cells and show that this results in a decrease in levels of ribosomal S6 phosphorylation and HTT expression ... More