Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis> | … Methylated substrates biotin - 5′ - UCAACm 6 AGC - 3′ (GenScript, Nanjing, China); Nicotinamide adenine dinucleotide (NAD + ), α- ketoglutaric acid, L-ascorbic acid and HEPES (Sigma Aldrich, Wisconsin, USA); AlphaScreen Protein A kit (PerkinElmer, Connecticut, USA … | Get A Quote |
N6-methyladenosine (mA) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). Until now, two RNA demethylases have been identified, including FTO (fat mass and obesity-associated protein) and ALKBH5 (α-ketoglutarate-dependent dioxygenase alkB homologue 5). As a mammalian mA demethylase, ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles, and ALKBH5 may play a significant role in these biological processes. Nevertheless, no modulator of ALKBH5 has been reported. The reason for that may be the lack of in vitro assays for ALKBH5 inhibitor screening. Herein, we describe the development of two homog... More