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Production of a mono-biotinylated EGFR nanobody in the E. coli periplasm using the pET22b vector.

BMC Res Notes. 2018-10;

NoorAlfiah，WalserGudrun，WesselingMatthijs，GironPhilippe，LaffraAlbert-Menno，HaddouchiFatima，De GrèveJacques，KronenbergerP

Products/Services Used	Details	Operation
Gene Synthesis	… addition of extra biotin in the medium. The EL2BH synthetic DNA sequence was ordered from GenScript and subcloned into vector pET22b (EMDMillipore) between EcoRI and HindIII sites. The EL2BH nanobody was isolated from …	Get A Quote

摘要

Our aim was to produce a mono-biotinylated single domain antibody ('nanobody') specific for the epidermal growth factor receptor (EGFR)， which is overexpressed in many cancer cells. The binding of the nanobody and its function are tested in cancer cells. The construct could be used to carry variable therapeutic or diagnostic load using biotin-streptavidin bridging.