| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … plasmid pET32a-RbIFN-αNov1 was transformed into Rosetta (DE3) Lys cells, and the recombinant protein rRbIFN-αNov1 was induced with IPTG (Sigma-Aldrich, USA), which contained a His-Tag that can be purified on a nickel-chelated column (GenScript, Nanjing, China) … | Get A Quote |
The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1， which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1)， which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed， its antiviral activity， physicochemical characteristics， and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. ... More
