| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … the pFRT/LacZeo plasmid was cut using Xba1-Xma1 restriction sites to remove the β-Lac portion of the LacZeo fusion protein cassette and the removed sequence was replaced by a custom-made mCherry sequence (designed by DCS and synthesized by Genscript (NJ, USA) … | Get A Quote |
The well-characterized cell line Chinese hamster ovary (CHO) has been used to produce numerous biopharmaceuticals and is an important tool for basic research. However， introducing foreign DNA into specially modified CHO cells such as DG44 and Lec 3.2.8.1 can sometimes be an arduous process. Here we show that the Flp-in plasmid can be modified to produce a fluorescent tracer protein tag (mCherry) as a fusion reporter， to allow for the rapid selection of single-cell sorted， isogenic Flp-in-ready DG44 and Lec 3.2.8.1 cell lines. These two cell lines are stable and viable and may be useful for applications such as antibody production and crystallographic studies. Here we provide key details on how the modifie... More
