Products/Services Used | Details | Operation |
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DNA Sequencing> | … The DNA sequences of nadV genes of these two organisms obtained by synthesis (GenScript) were cloned in pET-28a+ vector while NAMPT from Mus musculus was conveniently available on pET-28a(+) E. coli expression vector at Addgene … | Get A Quote |
Diabetes is a chronic and progressive disease with continuously increasing prevalence, rising financial pressure on the worldwide healthcare systems. Recently, the insulin resistance, hallmark of type 2 diabetes, was cured in mice treated with NAD precursor β-nicotinamide mononucleotide (NMN), no toxic effects being reported. However, NMN has a high price tag, more cost effective production methods are needed. This study proposes a biotechnological NMN production method in Escherichia coli. We show that bicistronic expression of recombinant nicotinamide phosphoribosyl transferase (Nampt) and phosphoribosyl pyrophosphate (PRPP) synthetase in the presence of nicotinamide (NAM) and lactose may be a ... More