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Gene Synthesis> | … 2.2. Preparation of recombinant human IMPase The gene encoding human IMPase with an amino-terminal six-histidine tag separated by an HRV 3C protease cleavage site was synthesized and subcloned into a pET-15b vector by GenScript … | Get A Quote |
Lithium, which is still the gold standard in the treatment of bipolar disorder, has been proposed to inhibit inositol monophosphatase (IMPase) and is hypothesized to exert its therapeutic effects by attenuating phosphatidylinositol (PI) cell signalling. Drug-discovery efforts have focused on small-molecule lithium mimetics that would specifically inhibit IMPase without exhibiting the undesired side effects of lithium. L-690,330 is a potent bisphosphonate substrate-based inhibitor developed by Merck Sharp & Dohme. To aid future structure-based inhibitor design, determination of the exact binding mechanism of L-690,330 to IMPase was of interest. Here, the high-resolution X-ray structure of human IMPas... More