| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … Construction, cloning, and expression of PDC variants. DNA sequences for each PDC variant were codon optimized and cloned into a pET22b(+) vector (GenScript, Piscataway, NJ). The sequence for a hexahistidine tag was placed at the C terminus of the constructs … | Get A Quote |
Strategies for maximizing the microbial production of bio-based chemicals and fuels include eliminating branched points to streamline metabolic pathways. While this is often achieved by removing key enzymes， the introduction of nonnative enzymes can provide metabolic shortcuts， bypassing branched points to decrease the production of undesired side-products. Pyruvate decarboxylase (PDC) can provide such a shortcut in industrially promising thermophilic organisms; yet to date， this enzyme has not been found in any thermophilic organism. Incorporating nonnative enzymes into host organisms can be challenging in cases such as this， where the enzyme has evolved in a very different environment from that of the... More
