Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | … protease cleavage site in order to have the option for removing the affinity tag. The gene was synthesized and subcloned into pET24a(+) via NdeI and NotI by Genscript (USA), giving vector pET24-His(TV)-AroL-Ecoli(op). 2.2 Recombinant expression of AroL in E. coli … | Get A Quote |
Shikimic acid 3-phosphate, as a central metabolite of the shikimate pathway, is of high interest as enzyme substrate for 5-enolpyruvoyl-shikimate 3-phosphate synthase, a drug target in infectious diseases and a prime enzyme target for the herbicide glyphosate. As the important substrate shikimic acid 3-phosphate is only accessible via a chemical multi-step route, a new straightforward preparative one-step enzymatic phosphorylation of shikimate using a stable recombinant shikimate kinase has been developed for the selective phosphorylation of shikimate in the 3-position. Highly active shikimate kinase is produced by straightforward expression of a synthetic aroL gene in Escherichia coli. The time course ... More