Products/Services Used | Details | Operation |
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PCR and Cloning> | … plasmid ( FIG. 2 ). First, the rBet v 1 cDNAs designed in Example 1, with the codon optimization for Escherichia coli production in vector pUC57 (wt and N28K-E101K) were ordered from GenScript Corporation (USA). The cDNAs … | Get A Quote |
We previously identified an expressed sequence tag clone, Der f 22, showing 41% amino acid identity to published Der f 2, and show that both genes are possible paralogues. The objective of this study was to characterize the genomic, proteomic and immunological functions Der f 22 and Der f 2. The full-length sequence of Der f 2 and Der f 22 coded for mature proteins of 129 and 135 amino acids respectively, both containing 6 cysteine residues. Phylogenetic analysis of known group 2 allergens and their homologues from our expressed sequence tag library showed that Der f 22 is a paralogue of Der f 2. Both Der f 2 and Der f 22 were single gene products with one intron. Both allergens showed specific IgE-bi... More