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Gene Synthesis> | … 2.2. Gene synthesis and vector construction. The chimeric peptide, derived from lactoferrin of camel milk, encoding sequence (cLFchimera) [13] was codon optimized for proper expression in E.coli and chemically synthesized by Genscript ® (USA) … | Get A Quote |
Over the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric peptide derived from camel lactoferrin was produced in Escherichia coli periplasmic space using a pET-based expression system and its antibacterial activity was determined on some avian pathogens in vitro. A carboxy-terminal polyhistidine tag was used for purification... More