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Gene Synthesis> | … 2. Materials and methods. 2.1. Expression and purification of NS4A of DENV4. The cDNA encoding full-length NS4A of DENV-4 fused C-terminally with a hexahistidine (His) 6 tag was synthesized (Genscript) and cloned into the Nde I and XhoI sites of pET29b … | Get A Quote |
Dengue virus (DENV) non-structural (NS) 4A is a membrane protein essential for viral replication. The N-terminal region of NS4A contains several helices interacting with the cell membrane and the C-terminal region consists of three potential transmembrane regions. The secondary structure of the intact NS4A is not known as the previous structural studies were carried out on its fragments. In this study, we purified the full-length NS4A of DENV serotype 4 into dodecylphosphocholine (DPC) micelles. Solution NMR studies reveal that NS4A contains six helices in DPC micelles. The N-terminal three helices are amphipathic and interact with the membrane. The C-terminal three helices are embedded in micelles. Our resul... More